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buy priligy tablets An Applied Biosystems Thermal Cycler 2720 Life Technologies, USA was set up with a first cycle of denaturation for 5 min at 94 C, followed by 35 cycles of denaturation at 94 C for 1 min, annealing at 60 C for 1 min, elongation at 72 C for 1 min, and a final extension step of 7 min at 72 C

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